• GenUP™ Plasmid Kit

GenUP™ Plasmid Kit

  • Biotechrabbit
  • BR0700200
  • In Stock
  • 740,00Kč

Dostupné možnosti

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Tento výrobek má minimální množství 1

room temperature

Features

  • Universal kit for both high- and low-copy-number plasmids isolation
  • Fast and simple procedure
  • High yields of pure plasmid DNA suitable for all applications

Applications

  • Plasmid DNA isolation from Gram-negative and Gram-positive bacteria

Purchase GenUP™ Plasmid Kit

Availability: In stock

Catalog #SizePackage Content
BR070020110 prepsSolutions, Mini Filters and Vials

BR070020250 prepsSolutions, Mini Filters and Vials

BR0700203250 prepsSolutions, Mini Filters and Vials

Biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.

The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.


STARTING MATERIAL

Bacterial cultures: 0.5–15 ml for high-copy-number plasmids, low-copy-number plasmids or cosmids

EXTRACTION TIME

Approximately 15 min

BINDING CAPACITY

Approximately 80 µg plasmid DNA

TYPICAL YIELD

Variable: 45–60 µg high-copy plasmid DNA is typically purified from 15 ml culture, or 6–20 µg from 2 ml culture 

AVERAGE PURITY

A260/A280 1.7–2.0


product manual